Arriving from the north:

Motorway A14 Adriatica
Take the Cesena Nord exit (150 km to Assisi), then continue in the direction of Perugia (E45), getting off at the exit for Assisi (SS75).

Motorway A1 del Sole
Take the Valdichiana exit, until you reach Perugia, then continue on to Cesena (E45), getting off at the exit for Assisi (SS75).

Arriving from the south:

Motorway A14 Adriatica
Take the Civitanova Marche exit, then continue in the direction of Foligno-Perugia getting off at the exit for Assisi (SS75).

Motorway A1 del Sole
Take the Orte exit, then continue in the direction of Perugia-Cesena (E45), getting off at the exit for Assisi (SS75).

Once you've arrived in Assisi, go to Piazza Matteotti and then leave the walls of the town through Porta Perlici Arch; go ahead for about 5 km towards Gualdo Tadino; turn on the right following the sign "Agriturismo La Castellana" (ignore the sign "Costa di Trex") and go ahead for about 500 m in the main road.